Physiological and Biochemical Effect of Sodium Chloride (NaCl) Induced Salinity Stress on Three Cultivars of Radish
Sheetal Sharma, Nisha K, V. D. Mendhulkar
Antioxidant, Catalases, Dismutases, Electrolyte leakage, Lipid Peroxidation, Peroxidases, Proline, Superoxide.
Salinity, is a major environmental factor that adversely affects plant growth and development and limits agricultural production. A very effective and simple screening technique for the identification of salt-tolerant and salt-sensitive radish genotypes was observed. In the present study, the effect of increasing NaCl concentrations was investigated on three varieties (Pusa chetki, Mino early long, and Red round radish) of Raphanus sativus.L. Seedlings were grown and irrigated with distilled water for 20 days and thereafter treated with different NaCl concentrations (0, 25, 75, 125 and 175mM) for 20 days. The relative tolerance of selected varieties was studied by analyzing differences in various parameters like Germination percentage, Seedling vigor index, Electrolyte leakage, Relative water content, Total chlorophyll content, Proline, Protein, Lipid Peroxidation, and Antioxidant enzymatic assays (Catalase Activity, Peroxidases Activity, and Superoxide Dismutases Activity). Physiological and biochemical properties were reduced significantly by the induction of salinity stress in all cultivars. Germination percentage, Leaf relative water content, Malondialdehyde concentration, Total chlorophyll content, Proline concentration, Protein content, and Enzymatic activity of Pusa chetki were determined to be higher than Red round radish and Mino early long Cultivars. Electrolyte leakage value was higher in the Mino Early Long cultivar than that in Red Round Radish and Pusa chetki. Our results showed that among all the three genotypes, Pusa Chetki and Red Round Ra Red round radish are salt-tolerant and Mino early long is a salt-sensitive genotype.
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Unique Paper ID: 154346

Publication Volume & Issue: Volume 8, Issue 10

Page(s): 627 - 636
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